Transferring a Gene Of Interest to a Suitable Vector & Host
There are many ways to insert a gene into a host and a vector. Cloning refers to the process of isolating a gene and inserting it into a vector (plasmid). Then, it’s possible to transfer that gene into an organism such as bacteria, yeast or insect cells (Khan and Ahmad 2020; Sun and al. 2020). The process involves extracting the desired gene from the source organism, and then inserting it in a vector. Once the vector has been transferred, the gene of your choice is activated in the target host cell. For example, the bacterial expression vector pET-281a can be used to recombinantly express genes in Escherichia coli. After the host and vector have been selected, the gene that is of most interest must be ligated to the vector. The restriction enzymes required to recognize the vector and cut it at certain recognition points (Sun et. al. 2020). Next, you can insert the gene of your choice into the vector by ligation. The recombinant gene is then transferred to the host by a suitable technique (conjugation transformation, transfection and so forth) (Chen, 2021). Cont…
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