Influence of Substrate Concentration On Enzyme Activity
It is proteins that are responsible for catalyzing biochemical reactions. The concentration of substrate affects the enzyme’s activity. The reaction speed increases when the substrate amount is greater than that of enzyme. However, once the substrate concentration reaches saturation, or maximum activity, further increases will not cause an increase in reaction rate. The enzyme molecules are saturated with substrate and have no active sites that can catalyze the reaction. (Ranjith 2020). This equation is commonly known as Michaelis-Menten equation and it is used widely to study the activity of enzymes at different substrate concentrations. (Larranaga. et. al., 2016). Michaelis-Menten’s equation says that at low substrate concentrations, the rate for the reaction is proportional to its concentration. Substance concentrations will also increase. As the enzyme becomes saturated, however, the reaction’s rate will not change if the substrate concentration is higher than the point at which it has reached saturation (Kumar and co-authors, 2018). This is known as the ‘saturation point’, and is an important concept in enzyme activity. Cont…..
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