Can Two DNA Molecules Kind Double-Stranded DNA (dsDNA)?
The formation of double-stranded DNA (dsDNA) depends on complementary base pairing between two strands of DNA, reminiscent of the 2 molecules in query, 5′-CCGATCGGAT-3′ and 5′-TCCGATC-3′. In accordance with a research by Liu and Zhang (2020), the 2 strands of DNA will need to have complementary bases to ensure that them to kind a bond and kind a double helix. Within the case of those two molecules, the bases will not be complementary, and thus, they won’t kind a dsDNA. Nevertheless, it’s potential to kind a dsDNA by ligation, which is the method of becoming a member of collectively two molecules of DNA. This course of may be performed in a laboratory setting and is usually used to affix two items of DNA that have been beforehand minimize aside (Aldrich et al., 2019). Within the case of those two molecules, the ends of the molecules would have to be modified in an effort to create complementary bases in order that they are often ligated collectively.
In conclusion, whereas these two molecules of DNA can’t kind a dsDNA on their very own, it’s potential to affix them collectively to kind a dsDNA via the method of ligation. This course of requires modification of the ends of the molecules in an effort to create complementary bases. Cont….
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