The Phenotype or LacI S of E. coli.
E. coli’s lac operon is composed of three genes: lacZ, lacY, and lacA. These three genes are controlled by the transcriptional repressor lacI. If the inducer protein lacI is not present, this protein will bind to the operator area of the operon. The organism’s phenotype would differ if a lacI S mutant (super-repressor) is present. Without the inducer the lac operon will remain inactive. The organism wouldn’t be capable of synthesising the enzymes required to metabolise Lactose. This would mean that it would be incapable to use lactose as a source of carbon. In laboratory experiments, this phenomenon was observed with E.coli that contains a lacI S variant, showing lactose utilization significantly lower than in wild-type E.coli cells (Dalton, et al. 2019,).
The inducer would also cause the lac operon to remain depressed. This would prevent organisms from synthesising the necessary enzymes for metabolising the inducer. Cont…
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