Create a Protein With a Polydistidine Label
Proteins are vital building blocks for life. They are also responsible for many important body functions. To facilitate manipulation and detection of proteins, one of the best ways to study them is by attaching a tag. The most common tag for protein research is the polydistidine tag. A number of steps are involved when creating a polydistidine protein. A gene encoded for the desired protein must first be inserted in an expression vector. The vector then transforms into a host-cell. Once the vector is created, the polydistidine tags are attached to the target protein. This tag typically contains three to five residues of histidine that link to the protein through a peptide bonds. The protein tagged by the tag is purified from its host cells using affinity or 2-D gel electrophoresis. After purification, you can study the tagged proteins using various laboratory methods.
Polydistidine tags have enjoyed increasing popularity in recent years because of their ease-of-purification and stability. Studies have shown that polydistidine tags exhibit high levels of stability over a wide range of temperatures (Vieira et al., 2016; Castillo-González et al., 2021). These tags can also be used to quickly and efficiently purify proteins from complex mixtures (Matsunaga, et al. 2019,). Cont….